HaloTag® Technology: A Powerful Tool for Protein Labeling and Analysis
Start by adding the HaloTag® protein fusion tag to your protein, pair the fusion protein with one of many interchangeable ligands that forms a highly specific covalent bond with HaloTag, and you open a gateway to investigate protein function. Once your protein of interest is fused to the HaloTag® protein, you are ready for numerous applications including protein purification, protein:protein or protein:DNA interactions, cellular imaging, receptor internalization and more. Each possible analysis pathway is based on which interchangeable HaloTag® ligand you select, or customize a ligand for your specific requirements.
![HaloTag Technology A Gateway for Protein Analysis Infographic](https://scv10mr-cdnpre-p-cus-00.azureedge.net/-/media/images/technology-pages/halotag-technology/halotag-technology-infographic-protein-analysis-gateway.png?rev=11c8297a937745dca6fd5cc2c4aff890&sc_lang=en)
Find Your HaloTag® Protein Analysis Tools
![HaloTag Protein Purification Technology](https://scv10mr-cdnpre-p-cus-00.azureedge.net/-/media/images/technology-pages/halotag-technology/halotag-icon-protein-purification.png?rev=f3f68a8806ee4e41a033849ce10c082a&sc_lang=en)
Protein Purification from E. coli and Mammalian Cells
Learn how to purify your protein fused to HaloTag from live cells and cell lysates.
![icon-imaging](https://scv10mr-cdnpre-p-cus-00.azureedge.net/-/media/images/technology-pages/nanoluc-technology/icon-imaging.png?rev=a31eace9e9f1409ab44b605c8e21ce7f&sc_lang=en)
Protein Localization, Imaging, Trafficking and Turnover
Track where proteins are in the cell, where they are going and how long they stay using fixed and live cells.
![HaloTag icon for PPI and Protein DNA Interactions](https://scv10mr-cdnpre-p-cus-00.azureedge.net/-/media/images/technology-pages/halotag-technology/halotag-icon-ppi-protein-dna-interactions.png?rev=fe5dc01c31424c1b96ebcd97dac3a110&sc_lang=en)
Protein:Protein and Protein:DNA Interaction Analysis
Study how proteins and protein complexes interact in live cells or examine DNA:protein interactions without antibodies.
![HaloTag icon for High Resolution Microscopy](https://scv10mr-cdnpre-p-cus-00.azureedge.net/-/media/images/technology-pages/halotag-technology/halotag-icon-high-resolution-microscopy.png?rev=ccb0a8a5bb8c4fdab2de425e04d146ce&sc_lang=en)
Super Resolution Microscopy
Follow a single molecule in cells when using a bright Janelia Fluor® ligand with a HaloTag® fusion protein.
![HaloTag icon for PPI and Protein DNA Interactions](https://scv10mr-cdnpre-p-cus-00.azureedge.net/-/media/images/technology-pages/halotag-technology/halotag-icon-ppi-protein-dna-interactions.png?rev=fe5dc01c31424c1b96ebcd97dac3a110&sc_lang=en)
HaloTag® Clones
Thousands of clones that have been validated by:
- Fluorescent imaging
- DNA sequencing
- Protein expression