HaloTag® Technology: Aqueous-Soluble Ligands Technical Manual
Instructions for Use of Product(s)
HT1010, HT1020, HT1030, HT1040, HT1050, HT1060, HT1070, HT1080, HT1090, HT1100, HT1110, HT1120, HT1130
Literature # TM753
The HaloTag® Technology platform addresses the need for flexibility in functional protein analysis. This modular technology is based on the formation of a covalent bond between a fusion tag, HaloTag® reporter protein, and synthetic ligands, and enables complete characterization of protein function in cellular and biochemical environments. There are a variety of HaloTag® Ligands and kits available for fluorescent cell imaging and protein capture/purification, as well as reactive customizable Ligand Building Blocks. The ligands described are soluble in cell media or aqueous buffers.
The modular nature of HaloTag® technology and the rapid binding kinetics with its ligands can directly image both protein trafficking and turnover. The available ligands have both varied fluorescent spectra and also cell-permeant and impermeant properties to provide spatial and temporal control. For example, you can label only the surface pool of a membrane-bound protein with a cell-impermeant ligand and follow its biology, internalization or other fate. Or a protein can first be labeled with a ligand that has one dye color, and after a period of time labeled again with a ligand containing a dye of a different color. Such pulse-chase labeling allows direct observation of protein trafficking and/or turnover over time.
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